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Test Code LAB7054   Parechovirus Detection by PCR

Performing Laboratory

 

Barnes-Jewish Hospital Molecular Infectious Disease Laboratory   

Methodology

Polymerase Chain Reaction (PCR) performed on the DiaSorin Liaison MDX.               

Specimen Requirements

Acceptable Specimens

CSF

  1. Aseptically collect ≥1 mL of CSF.
  2. Place in a leak-proof screw-capped, sterile container.
  3. Maintain sterility and forward promptly.
  4. If transport is delayed, refrigerate specimen.

 

Note:

  1. Specimen source is required.          

Day(s) Test Set Up

 

Monday through Sunday

Turnaround Time:
STAT: not available
Routine: 1- 3 days, Final report available the day of testing                     

Test Classification and CPT Coding

Test classification: 

This assay utilizes ASR’s (analyte specific reagents) and is not FDA cleared.  This test was developed and its performance has been evaluated by the Barnes Jewish Molecular Infectious Disease Laboratory for performance on CSF specimens.

 

Assay Limit of Detection:

Parechovirus Genotype 1 = 1,012 TCID50/mL

Parechovirus Genotype 2 = 202 TCID50/mL

Parechovirus Genotype 3 = 202 TCID50/mL

Parechovirus Genotype 4 = 202 TCID50/mL

Parechovirus Genotype 5 = 202 TCID50/mL

Parechovirus Genotype 6 = 202 TCID50/mL

 

Limitations:

  1. The detection of viral nucleic acid is dependent upon proper sample collection, transport, handling and storage. Failure to observe proper procedures in any one of these steps can lead to incorrect results.
  2. False-negative results may occur if the virus is present at a level that is below the analytical sensitivity of the assay or if the virus has genomic mutations, insertions, deletions, or rearrangements or if performed very early in the course of illness.

 

CPT Code:

87798

Reference Values

Negative

Additional Information

For BJH Laboratory Use Only

 

Minimum Volume: 
CSF: 0.5 mL

 Laboratory Processing Instructions:    

 BJH Microbiology will forward to the performing laboratory.  

Special Instructions

Specimen Transport Instructions

CSF-Ambient/Refrigerate/Frozen OK

Refrigerated specimens must be tested within 7 days
Frozen specimens must be tested within 30 days