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Test Code LAB713 Cryoglobulin and Cryofibrinogen Panel, Serum and Plasma

Additional Codes

Mayo Test ID
CRGSP

Reporting Name

Cryo Panel, S and P

Useful For

Evaluating patients with vasculitis, glomerulonephritis, and lymphoproliferative diseases

 

Evaluating patients with macroglobulinemia or myeloma in whom symptoms occur with cold exposure

 

This test is not useful for general screening of a population without a clinical suspicion of cryoglobulinemia.

Profile Information

Test ID Reporting Name Available Separately Always Performed
CRY_S Cryoglobulin, S Yes Yes
CRY_P Cryofibrinogen, P No Yes

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IMFXC Immunofixation Cryoglobulin No No

Testing Algorithm

If cryoglobulin has a positive result after 1 or 7 days, then immunofixation will be performed at an additional charge. Positive cryoglobulins of 0.1 mL or above of precipitate will be typed once.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Plasma EDTA
Serum Red


Specimen Required


Both plasma and serum are required.

 

Cryofibrinogen

Collection Container/Tube: Lavender top (EDTA)

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Tube must remain at 37° C.

2. Centrifuge at 37° C. (Do not use a refrigerated centrifuge. If absolutely necessary, ambient temperature is acceptable.) It is very important that the specimen remain at 37° C until after separation of plasma from red blood cells.

3. Place plasma into an appropriately labeled plastic vial.

 

Cryoglobulin

Collection Container/Tube: Red top (serum gel/SST are not acceptable)

Submission Container/Tube: Plastic vial

Specimen Volume: 5 mL

Collection Instructions:

1. Tube must remain at 37° C.

2. Allow blood to clot at 37° C.

3. Centrifuge at 37° C. (Do not use a refrigerated centrifuge. If absolutely necessary, ambient temperature is acceptable.) It is very important that the specimen remain at 37° C until after separation of serum from red blood cells.

4. Place serum into an appropriately labeled plastic vial.

Additional Information: Analysis cannot be performed with less than 3 mL of serum. Smaller volumes are insufficient to detect clinically important trace (mixed) cryoglobulins. Less than 3 mL will require collection and submission a new specimen.


Barnes-Jewish Hospital Note:

ATTENTION: Serum cryoglobulin is available separately.

If specimen is serum only, cryoglobulin will be performed.

 

Cryoglobulin Only 
Specimen Type: Serum

Container/Tube: Plain red top

Specimen Volume: 3 full tubes
Collection Instructions: Prewarm tubes to 37° C

 

Collection Procedure:
Hospital Patient:
Obtain prewarmed tubes and prewarmed blue, insulated bag from Barnes-Jewish Hospital Chemistry Laboratory or North Campus Laboratory.

1. Draw specimen using 3 prewarmed tubes.
2. Immediately place into prewarmed insulated bag and zip closed immediately.
3. Deliver immediately to Chemistry Laboratory.
Additional Information:

1. Temperature of specimen must be between 32° C to 42° C upon receipt. Specimens not received at proper temperature will be rejected.
2. Analysis cannot be performed with <3 mL of serum. Smaller volumes are insufficient to detect clinically important trace (mixed) cryoglobulins. Less than 3 mL will require a new specimen be drawn.
 
Non Hospital Patient:
Call Barnes-Jewish Hospital Laboratory Customer Service at 314-362-1470 prior to collection for processing instructions.
 
Cryoglobulin and Cryofibrinogen
Hospital Patient:
Obtain prewarmed tubes and prewarmed blue, insulated bag from Barnes-Jewish Hospital Chemistry Laboratory or North Campus Laboratory.
 
Plasma and serum are required.
 
Plasma for Cryofibrinogen
Specimen Type: Plasma

Container/Tube: Lavender top (EDTA)

Specimen Volume: 1 full tube
Collection Instructions: Prewarm to 37° C.
 
Collection Procedure:
Hospital Patient:
1. Draw specimen using 1 prewarmed tube.
2. Immediately place into prewarmed insulated bag and zip closed immediately.
3. Deliver immediately to Chemistry Laboratory.
Additional Information: Temperature of specimen must be between 32° C to 42° C upon receipt. Specimens not received at proper temperature will be rejected.
 
Non Hospital Patient:
Call Barnes-Jewish Hospital Laboratory Customer Service at 314-362-1470 prior to collection for processing instructions.
 
Serum for Cryoglobulin
Specimen Type: Serum

Container/Tube: Plain red top

Specimen Volume: 3 full tubes
Collection Instructions: Prewarm tubes to 37° C

 

Collection Procedure:
Hospital Patient:
Obtain prewarmed tubes and prewarmed blue, insulated bag from Barnes-Jewish Hospital Chemistry Laboratory or North Campus Laboratory.
 
1. Draw specimen using 3 prewarmed tubes.
2. Immediately place into prewarmed insulated bag and zip closed immediately.
3. Deliver immediately to Chemistry Laboratory.
Additional Information:

1. Temperature of specimen must be between 32° C to 42° C upon receipt. Specimens not received at proper temperature will be rejected.
2. Analysis cannot be performed with <3 mL of serum. Smaller volumes are insufficient to detect clinically important trace (mixed) cryoglobulins. Less than 3 mL will require a new specimen be drawn.
 
Non Hospital Patient:
Call Barnes-Jewish Hospital Laboratory Customer Service at 314-362-1470 prior to collection for processing instructions.

 

CPT Codes:

82595-Cryoglobulin

86334-Immunofixation (if appropriate)

If specimen is plasma and serum, cryoglobulin and cryofibrinogen will be performed.

CPT Codes:

82585-Cryofibrinogen

82595-Cryoglobulin

86334-Immunofixation (if appropriate)

 

Specimen Minimum Volume

Plasma: 0.5 mL
Serum: 3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma EDTA Refrigerated (preferred)
  Frozen 
Serum Red Refrigerated (preferred)
  Frozen 

Reference Values

CRYOGLOBULIN

Negative (positives reported as percent or trace amount)

If positive after 1 or 7 days, immunotyping of the cryoprecipitate is performed at an additional charge.

 

CRYOFIBRINOGEN

Negative

Quantitation and immunotyping will not be performed on positive cryofibrinogen.

Day(s) Performed

Monday through Friday

CPT Code Information

82585

82595

86334-Immunofixation (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CRGSP Cryo Panel, S and P 74352-6

 

Result ID Test Result Name Result LOINC Value
2685 Cryofibrinogen, P 11043-7
2684 Cryoglobulin, S 12201-0

Report Available

2 to 10 days

Method Name

CRY_S, CRY_P: Quantitation and Qualitative Typing Precipitation

IMFXC: Immunofixation

Forms

If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen.

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
Barnes-Jewish Hospital Additional Information:

 

For BJH Laboratory Use Only
Analyte Stability:Specimen is stable indefinitely after processing at 2° C to 8° C.
Laboratory Processing Instructions:
Deliver specimen to BJH Chemistry for processing. 

 

Note: Serum cryoglobulin is available separately. If serum only is received, process specimen and forward to Chemistry for send out to Mayo. Take the temperature of the blood; acceptable range is 32° C to 42° C. Record temperature in the computer. Allow the tubes to clot for 1 hour at 37° C. Spin the tubes in a prewarmed centrifuge (37° C) for 10 minutes at 3,000 rpms. Pipette the cell-free serum into a 15-mL graduated, conical, screw-top tube. Label tube with computer label. Store in the refrigerator (2° C-8° C) before testing.