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Test Code LAB9788 BAL, T-Cell Subset

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Methodology

Profile Information:
CD3
CD4
CD8
CD4/CD8 Ratio

Method Name:

Flow Cytometry

Literature References:

1.  Heron M, Grutters JC, ten Dam-Molenkamp KM, Hijdra D, van Heugten-Roeling A, Claessen AM, Ruven HJ, van den Bosch JM, van Velzen-Blad H. Bronchoalveolar lavage cell pattern from healthy human lung. Clin Exp Immunol. 2012 Mar;167(3):523-31. doi: 10.1111/j.1365-2249.2011.04529.x. PMID: 22288596; PMCID: PMC3374285.

2.  Harbeck R. J. (1998). Immunophenotyping of bronchoalveolar lavage lymphocytes. Clinical and diagnostic laboratory immunology, 5(3), 271–277. https://doi.org/10.1128/CDLI.5.3.271-277.1998

Specimen Requirements

Specimen must arrive within 72 hours of draw. Specimen must arrive by 1600 on Friday and 1 day before a holiday.

 

Specimen Type: BAL Fluid

Container/Tube: Sterile container

Specimen Minimum Volume: 1 mL

Additional Information: Specimen cannot be refrigerated.

Day(s) Test Set Up

Monday through Friday

Cut-off time: 2100 Monday through Thursday; 1600 Friday and eve before a holiday

Turnaround Time:
STAT: not available
Routine: 48 hours

Test Classification and CPT Coding

86355-B-cells, total count

86359-T-cells, total count

86360-T-cells CD4+CD8 with ratio

Reference Values

CD4/CD8 RATIO

0.6-5.5
 

  Total Cells
 (CD3)		 Helper Cells
 (CD4)		 Cytotoxic
T-Cells (CD8)
Adult      
Percentage: 90%-98% 35%-79% 15%-57%

 

Specimen Transport Temperature

Ambient/Refrigerate NO/Frozen NO

Additional Information

For BJH Laboratory Use Only
Alternate Tube:

Analyte Stability:

Specimen cannot be refrigerated. Specimen must be processed within 72 hours of draw and kept at ambient temperature.

Laboratory Processing Instructions:
Test performed in BJH Flow Cytometry.