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Test Code Test Code LAB7016 Tissue and Bone Culture  

Infectious

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Methodology

Gram stains are routinely performed on the following specimens: aspirate, bone, lymph node, tissue. Because culture is more sensitive than Gram stain, the Gram stain is only offered in combination with a culture request.

Specimen Requirements

Acceptable Specimens:

≥1g in leakproof, screw-capped, sterile container, or >1g in anaerobic transport device

 

Collection Procedure:
1. Aseptically collect ≥2 g of specimen and place >1g in a leakproof, screw-capped, sterile container and >1g in an anaerobic transport device. Because there may be rare viable organisms, specimens must be large enough to permit their recovery.
2. Maintain sterility and forward promptly.

Day(s) Test Set Up

Monday through Sunday

Test Classification and CPT Coding

87070-Culture, aerobic (not stool, rectal swab, or urine) (if appropriate)
87181-Susceptibility, agar diffusion (if appropriate)
87184-Susceptibility, disk diffusion (if appropriate)
87185-Susceptibility, enzyme detection (if appropriate)
87186-Susceptibility, microdilution (if appropriate)
87205-Gram stain (if appropriate)

Reference Values

Not applicable

 

Literature Reference:

Carroll, Karen, et. al. Manual of Clinical Microbiology, 13th edition. ASM Press. Washington, D.C. 2023

Specimen Transport Temperature

Ambient

Additional Information

Minimum Volume:
1g in leakproof, screw-capped, sterile container and 1g in anaerobic transport device.

Laboratory Processing Instructions:
Test performed in BJH Microbiology.