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Test Code LAB264 (FGEN) Mold (fungal) culture, Bone Marrow

Performing Laboratory

Mayo Clinical Laboratory

Methodology

Specimens are cultured on selective fungal media (eg, inhibitory mold agar and brain heart infusion blood agar with chloramphenicol and gentamicin). Respiratory sources also are cultured on brain heart infusion agar with chloramphenicol, gentamicin, and cycloheximide. Cultures are incubated for 24 days at 30° C.

 

Identification of fungi is based on colonial and microscopic morphology, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, laboratory-developed real-time polymerase chain reaction assays and/or D2 ribosomal RNA gene sequencing, as applicable.

Specimen Requirements

Acceptable Specimens:

 

> 3mL of bone marrow collected in green top lithium heparin tube.

 

 

Collection Procedure:
1. Disinfect lithium heparin tube stopper with alcohol prep pad. Let dry completely.
2. Aseptically collect > 3mL of bone marrow into lithium heparin tube.
3. Maintain sterility and forward promptly at ambient temperature only.

 

Click here for collection instruction flier.

Day(s) Test Set Up

Monday through Sunday

 

Turnaround Time:
STAT: not available
Routine: report available in 24 to 35 days

Test Classification and CPT Coding

87102 – Fungal culture, routine

87106 – Id MALDI-TOF Mass Spec Yeast (if appropriate)

87107 – Id MALDI-TOF Mass Spec Fungi (if appropriate)

87107 – Fungal identification panel A (if appropriate)

87107 – Fungal identification panel B (if appropriate)

87150 x 2 – Identification Histoplasma / Blastomyces, PCR (if appropriate)

87153 – D2 fungal sequencing identification (if appropriate)

87150 – Id, Candida auris rapid PCR (if appropriate)

Reference Values

Not applicable

 

Literature Reference:

Carroll, Karen, et. al. Manual of Clinical Microbiology, 13th edition. ASM Press. Washington, D.C. 2023

Sample Transport Temperature

Ambient/Refrigerate NO/Frozen NO

Additional Information

For BJH Laboratory Use Only
Minimum Volume: 1 mL

 


Laboratory Processing Instructions:
Test performed at Mayo Clinical Laboratory.