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Test Code LAB270 (CTB) Mycobacteria Culture, Bone Marrow

Performing Laboratory

Mayo Clinic Laboratory

Methodology

This culture is useful for the detection and identification of Mycobacterium species from bone marrow specimens.  

 

For mycobacterial culture, bone marrow specimens are inoculated to both liquid and solid media. The BACTEC MGIT 960 System is designed for the rapid detection of mycobacteria in clinical specimens. The automated BACTEC MGIT 960 System monitors the tubes hourly for increasing fluorescence. Analysis of the fluorescence is used to determine if the tube is instrument-positive, ie, the test contains viable organisms. Culture tubes that remain negative for a minimum of 42 days and that show no visible signs of positivity are removed from the instrument as negatives. In addition to the MGIT tube, Middlebrook 7H10/7H10S agar biplates are inoculated and incubated at 37° C. Growth from positive MGIT tubes or agar plates is identified using a variety of techniques matrix assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry or 16S rRNA gene sequencing.

Specimen Requirements

Acceptable Specimens:

 

> 3mL of bone marrow collected in green top lithium heparin tube

 

 

Collection Procedure:
1. Disinfect lithium heparin tube stopper with alcohol prep pad. Let dry completely.
2. Aseptically collect > 3mL of bone marrow into lithium heparin tube.
3. Maintain sterility and forward promptly at ambient temperature only. 

 

Click here for collection instruction flier.

Day(s) Test Set Up

Monday through Sunday

 

Turnaround Time:
STAT: not available
Culture: a final negative is issued after 42 days of incubation.  Positive cultures are reported as soon as detected.

Test Classification and CPT Coding

87116 – Mycobacterial culture

87118 – ID MALDI-TOF Mass Spec AFB (if appropriate)

87150 – Mycobacteria Probe Ident (if appropriate)

87150 – Id, MTB Speciation, PCR (if appropriate)

87153 – Mycobacteria Identification by Sequencing (if appropriate)

Reference Values

Not applicable

Sample Transport Temperature

Ambient/Refrigerate NO/Frozen NO

Additional Information

For BJH Laboratory Use Only
Minimum Volume: 1.5 mL

 


Laboratory Processing Instructions:
Test performed at Mayo Clinical Laboratory.