Test Code LAB3618 Syphilis Testing for Disease/Treatment Monitoring

Infectious

Performing Laboratory

Barnes-Jewish Hospital

Particle Agglutination

Testing Algorithm:

Qualitative RPR is performed and if Positive, RPR Titer will be performed at an additional charge.

Literature References:

Larsen SA, Hunter EF, Kraus SJ. A manual of tests for syphilis. Washington, DC: American Public Health Association, 1990.
Alere IMPACT Rapid Plasmin Reagin (RPR) Card Test Package Insert, Alere North America LLC, 30 South Keller Rd., Orlando, FL 32810 11/12/2015.
Papp JR, Park IU, Fakile Y, Pereira L, Pillay A, Bolan GA. CDC Laboratory Recommendations for Syphilis Testing, United States, 2024. MMWR Recomm Rep. 2024 Feb 8;73(1):1-32.

Specimen Type: Serum

Container/Tube: Plain red top, Red/Grey or Gold top preferred

Specimen Volume: Full tube, 1mL minimum

Monday through Sunday
Turnaround Time:
Stat: ED, LD ordered STAT – 2 hours

Routine: Same Day

Reference: Negative

RPR reported as Negative, Positive

RPR Titer reported as doubling dilution ratio 1:1, 1:2, 1:4, 1:8, 1:16, etc.

Ambient

For BJH Laboratory Use Only

Additional specimens: EDTA Lavender top only for children <18 months.
Laboratory Processing Instructions:

Specimens will be put on the Cobas automation line to centrifuge, then go to MAN1-S1-OBS1 rack to be delivered to Setups bench.