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Test Code LAB450 Uric Acid, Urine, Timed

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Methodology

Enzymatic (Uricase)

Literature Reference:

Package insert: Uric acid UA2. Roche Corp., 12/2018.

Specimen Requirements

Specimen Type: Urine, timed

Container/Tube: Plain, brown urine container-Specimen collected in a container with an acid or base is not acceptable.

Specimen Volume: Entire collection

 

Collection Instructions: No additive. Refrigerate specimen during collection.

Additional Information:

1. Starting and ending times of collection are required.

2. See Urine Collection information under General Information.

3. Containers with preservatives are NOT acceptable.

Day(s) Test Set Up

Monday through Sunday
Turnaround Time:
STAT: 1 hour after receipt in laboratory
Routine: 2 to 4 hours after receipt in laboratory

Test Classification and CPT Coding

81050-Volume measurement for timed collection

84560-Uric acid

Reference Values

No established reference intervals

Literature Reference:

Rifai N, Horvath AR, Wittwer AT eds: Textbook of Clinical Chemistry and Molecular Diagnostics 6 th ed: Chapter 32, p. 479, Elsevier, St. Louis, MO 2018.

Specimen Transport Temperature

Ambient

Additional Information

For BJH Laboratory Use Only

Analyte Stability:

Specimen must be kept refrigerated during collection and before delivery to limit bacterial growth.

Laboratory Processing Instructions:

Test performed in BJH Core Lab. Mix urine well. Record volume, period, and pH on request form. Using 13x100 test tubes, prepare a 7-mL aliquot. Check the pH. If pH is <9, add 6 N NaOH in 1 drop increments until the pH is between 9 and 10. Do not over adjust. Heat for 10 minutes at 56° C. Deliver to the automated line for centrifugation and delivery to Cobas 8000 for analysis. Completed specimen is stored refrigerated for 5 days.

Special Instructions

See Urine Collection information under General Information.

See Analyte Stability Chart under General Information for a complete list.