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Test Code LAB4922 Chromogenic Factor VIII

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Methodology

Spectrophotometric measurement

 

Literature References:

Cryocheck™ Chromogenic Factor VIII (REF CCF08) package insert issued 2022, Precision BioLogic

Specimen Requirements

Specimen Type: Plasma

Container/Tube: Light-blue top (citrate)

Specimen Volume: Full tube

Specimen Minimum Volume: Full tube

Collection Instructions:

 

  1. Draw a discard blue top tube prior to drawing a light-blue top (citrated) tube if using a Winged Infusion Set.  Discard tube should be under filled and discarded.
  2. Clotted blood or an insufficient specimen is not acceptable.
  3. Avoid phlebotomy techniques which cause hemolysis and heparin contamination.
  4. Follow nursing policy on volume of blood to discard prior to collecting blood from indwelling heparinized catheters.

Analyte Stability:

2 hours at 18°C to 24°C.

Specimen cannot be refrigerated.

Platelet free plasma may be stored up to 3 months at -70°C.

 

Additional information: If the patient is a hospital patient and the hematocrit is >55%, call Barnes-Jewish Hematology Laboratory at 314-362-5203 for instructions.

Test Classification and CPT Coding

85130

Reference Values

43-160%

 

Literature References:

1. ACL TOP On-Line Help Manual Rev 2.2, Instrumentation Laboratory.

2. Precision BioLogics www.precisionbiologic.com

3. Marlar R, Strandberg K, Shima M, Adock DM. Clinical utility and impact of the use of the chromogenic vs one-stage factor activity assays in haemophilia A and B. Eur J Haematol. 2020 Jan;104(1):3-14.

Specimen Transport Temperature

Ambient Yes/Refrigerate NO/Frozen platelet free plasma

Additional Information

For BJH Laboratory Use Only

Analyte Stability:

2 hours at 18°C to 24°C.

Specimen cannot be refrigerated.

Platelet free plasma is stable for 3 months at -70°C.

 

Laboratory Processing Instructions:

Test performed in BJH Core Lab.

Day (s) Test Set Up

Monday, Wednesday, Friday

STAT: Requires LMR approval

Routine: 1-3 days after receipt in the laboratory

LOINC Code Information

Name

LOINC Code

Chromogenic Factor VIII

49865-9