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Test Code LAB5029 Amikacin Level (Trough, Random, or Peak)

Performing Laboratory

St. Louis Children's Hospital

Last Reviewed

November 2024

Lab Dept

Core Lab Chemistry

Specimen Requirements

Specimen Type: Blood

Container/Tube  
Preferred: Light green-top (Lithium heparin) Vacutainer
Acceptable: Plain, red-top Vacutainer or Lavender-top (EDTA) Vacutainer

Collection Volume: 2.0 mL (minimum 1.0 mL)
Patient Preparation: None
Sample Rejection: Mislabeled or unlabeled specimens.
Special Instructions: Trough Specimen: Collect immediately before next scheduled dose or no earlier than 1 hour prior to dose. (Trough level for first dose should be collected just prior to next dose).

Peak Specimen: Collect 30-60 minutes after dose.

Send to lab promptly.

General Information

EPIC order name:

Amikacin Level, Trough (EPIC Test # LAB5029)

Amikacin Level, Random (EPIC Test# LAB145)

Amikacin Level, Peak (EPIC Test# LAB5028)

Cerner order name:

Amikacin Tr

Amikacin Rnd

Amikacin Pk

CPT Codes: 80150
LOINC Value:

Amikacin Tr: 3321-7

Amikacin Rnd: 35669-1

Amikacin Pk: 3319-1

Synonyms:  

Interpretive

Therapeutic Range: ranges apply to all ages

Trough Level: <8.0 mcg/mL

Random Level: No established reference interval

Peak Level: 10.0-35.0 mcg/mL

Critical Values:

Random level: ≥75.0 mcg/mL

Trough level: >8.0 mcg/mL

Critical test results will be automatically called back initially and every 24 hours thereafter.
Limitations: NA
Methodology: Immunoassay (Kinetic interaction of microparticles in solution (KIMS) as measured by changes in light transmission).

Additional Information

For SLCH Laboratory use only

Lab Processing instructions: Centrifuge specimen to separate. If specimen is collected in a plain, red-top or Lavender-top (EDTA) tube; aliquot sample into a conical tube for testing (labeling with specimen type).
Processed Volume: 0.3 mL plasma/serum
Analyte Stability:

Ambient - unprocessed: 8 hours

PROCESSED:

Ambient: 8 hours
Refrigerated: 2 days
Frozen: 4 weeks
Notes: None

Logistics

Test Availability: Monday-Sunday
Cut-off time: None; performed as received
Turnaround Time: Same day; 2 hours from receipt in lab
Lab Testing Section: Chemistry

Barnes-Jewish Hospital Additional Information:

 

 

For BJH Laboratory Use Only
Laboratory Processing Instructions:BJH Chemistry will forward to the performing laboratory.

 

Stability: 8 hours room temperature, refrigerated plasma 2 days.