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Test Code LAB535 Vitamin D, 25-Hydroxy

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Methodology

Electrochemiluminescence Immunoassay (ECLIA)

Note: Do not confuse this test with the less commonly ordered #8000 1,25-Dihydroxyvitamin D, Serum.

Literature Reference:

Package insert: Vitamin D III Roche Corporation 11/2021.

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Specimen Requirements

Specimen Type: Plasma
Container/Tube: Mint Green top tube
Specimen Volume: Full tube

 

Day(s) Test Set Up

Monday through Sunday

Turnaround Time:
STAT: not available
Routine: results available the day of testing

Test Classification and CPT Coding

82306

Reference Values

30-80 ng/mL

This reference interval is consistent with recent published reports and the National Kidney Foundation’s Kidney Disease Outcomes Quality Initiative.

Literature References:

1. Holick MF. NEJM 2007;357:266-81.
2. K/DOQI Clinical Practice Guidelines for Bone Metabolism and Disease in Chronic Kidney Disease. kidney.org/professionals/KDOQI/guidelines_bone/Guide7.htm

Specimen Transport Temperature

Ambient/Refrigerate 24 hours OK

Additional Information

For BJH Laboratory Use Only

Alternate Tube(s):

Plain Red top tube

Dark green top (LiHep)

Purple top (K2 & K3 EDTA)

Analyte Stability:

See Analyte Stability Chart.
Laboratory Processing Instructions:

Test performed in BJH Core lab. Processed and aliquoted by Cobas 8100 automated line for delivery to the Cobas 8000. Completed specimens are stored refrigerated for 5 days.

Test performed in Ambulatory Cancer Building Lab.  Centrifuge sample and deliver to the Cobas.  Completed specimens are stored refrigerated for 5 days. 

LOINC Code Information

Name LOINC Code
Vitamin D, 25-Hydroxy, Serum 1989-3