Sign in →

Test Code LAB5618 ADAMTS-13 Evaluation,citrate plasma

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Methodology

FRET

Literature Reference:

Package insert: Technoclone Herstellung Von Diagnostika and Arzenemitteln, Technofluor ADAMTS13 Activity, 01/31/2024, Vienna, Austria.

Specimen Requirements

Specimen Type: Plasma

Container/Tube: Citrate (light blue)

Specimen Volume: Full tube

EDTA acts a a strong inhibitor of ADAMTS-13 and cannot be used.

Day(s) Test Set Up

Testing available Monday through Friday from 07:00-14:30.

 

Routine: Specimens must be recieved in lab by 14:30 p.m. each day.  Samples recieved after 14:30 will be run the next day.  Samples received after 14:30 on Friday will be run on Monday.

 

STAT: Samples may be run on evening shift or on the weekend, only with approval from the Hematology or Transfusion Medicine LMR (314-747-1320), option 5.

Test Classification and CPT Coding

85397 ADAMTS-13 Activity

85335 Reflex ADAMTS13 Inhibitor(LAB5620)

83520 Reflex ADAMTS-13 Antibody(LAB5619)

 

If ADAMTS13 Activity is <20% per inpatient hospital admission, a Reflex ADAMTS13 Inhibitor will be added on and sent to Verisiti Lab at an additional charge.

If the ADAMTS-13 inhibitor is negative (≤0.4) or low positive (0.4-0.7), Versiti will perform a Reflex ADAMTS-13 Antibody test.

 

 

 

Reference Values

Percent Activity >60% 

The following Interpretation will appear with all testing. ADAMTS13 activity less than 10 percent may be associated with either inherited (Upshaw-Schulman Syndrome) or acquired thrombotic thrombocytopenic purpura (TTP).  A variety of medical conditions may result in a mild to moderate deficiency of ADAMTS13 activity. Recent plasma exchange therapy may increase the observed ADAMTS13 activity.

 This test was developed and its performance characteristics determined by Barnes Jewish Hospital Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions

Reflex ADAMTS-13 Inhibitor(LAB5620)  - Added automatically when Activity is <20%

Specimen Transport Temperature

Ambient

Additional Information

Transport temperature: Ambient

Analyte Stability:

Primary tube: 24 hours at room temperature.

Frozen: 7 days. Avoid more than 1 freeze/thaw cycle

Lab Processing Instructions:

Test performed in Coagulation on the Ceveron s100 Monday through Friday 07:00-14:30.  LMR Approval needed for STAT testing on evenings and weekends.  Citrate specimens should be delivered to Coag tech to be spun platelet free and frozen -20 C.

LOINC Code Information

93371-3    von Willebrand factor (vWf) cleaving protease actual/normal in Platelet poor plasma by Chromogenic method