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HelpTest Code LAB5618 ADAMTS-13 Evaluation,citrate plasma
Performing Laboratory
Barnes-Jewish Hospital Laboratory
Methodology
ELISA chromogenic test
Literature Reference:
Package insert: Technoclone Herstellung von Daignostika und Arzneimittein, Technozyme ADAMTS-13 Activity, 8/2/2021, Vienna, Austria.
Specimen Requirements
Specimen Type: Plasma
Container/Tube: Citrate (light blue)
Specimen Volume: Full tube
EDTA acts a a strong inhibitor of ADAMTS-13 and cannot be used.
Day(s) Test Set Up
Routine: Thursday 11am cutoff. Specimens must be recieved in the lab by 11am.
STAT: Stat testing available to BJH and SLCH patients only. All other HSO should send STAT test to Versiti through that laboratory’s regular send out process.
Stat testing at BJH and SLCH require LMR Approval. Call 314-747-1320, choose option 1 for the Transfusion Med LMR. Specimens must be in the lab by 11am. LMR should call Lab Customer Service with Approval. STAT Results available by 3:30pm
Test Classification and CPT Coding
85397 ADAMTS-13 Activity
85335 Reflex ADAMTS13 Inhibitor(LAB5620)
83520 Reflex ADAMTS-13 Antibody(LAB5619)
If ADAMTS13 Activity is <20% per inpatient hospital admission, a Reflex ADAMTS13 Inhibitor will be added on and sent to Verisiti Lab at an additional charge.
If the ADAMTS-13 inhibitor is negative (≤0.4) or low positive (0.4-0.7), Versiti will perform a Reflex ADAMTS-13 Antibody test.
Reference Values
Percent Activity ≥64%
The following Interpretation will appear with all testing. ADAMTS13 activity less than 10 percent may be associated with either inherited (Upshaw-Schulman Syndrome) or acquired thrombotic thrombocytopenic purpura (TTP). A variety of medical conditions may result in a mild to moderate deficiency of ADAMTS13 activity. Recent plasma exchange therapy may increase the observed ADAMTS13 activity.
This test was developed and its performance characteristics determined by Barnes Jewish Hospital Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions
Reflex ADAMTS-13 Inhibitor(LAB5620) - Added automatically when Activity is <20%
Specimen Transport Temperature
Ambient
Additional Information
Transport temperature: Ambient
Analyte Stability:
Primary tube: 24 hours at 2-8 C
Frozen: 7 days. Avoid more than 1 freeze/thaw cycle
Lab Processing Instructions:
Test performed in Immunology Lab on Inova Quantalyzer Thursday 11am. LMR Approval needed for STAT testing on other days. Citrate specimens should be delivered to Coag tech to be spun platelet free and frozen -20 C.
LOINC Code Information
93371-3 von Willebrand factor (vWf) cleaving protease actual/normal in Platelet poor plasma by Chromogenic method