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Test Code LAB5796 Epstein-Barr Virus (EBV) DNA Detection (Quantitative), plasma

Methodology

Testing is performed by real-time PCR on the cobas® 6800 platform using the cobas® EBV assay (Roche Molecular Systems, Inc.).    

Specimen Requirements

Specimen Type: Plasma.

Container/Tube: Lavender or pink top (EDTA).

Specimen Minimum Volume: Minimum of 2 mL of whole blood in a dedicated lavender or pink top (EDTA) tube.

 

**Facilities outside of BJH should submit a minimum of 1 mL of plasma.

 

Collection Instructions:

1.  Spin down and separate plasma from cells within 24 hours of collection.

2.  Plasma should be stored at 2-8°C or -20°C upon separation.  Please see Specimen Stability Information for additional information.

Day(s) Test Set Up

Monday, Wednesday, Friday

Turnaround Time (STAT, Routine)    

STAT: Director approval required.

Routine: 24 – 48 hours       

Test Classification and CPT Code

Test Classification:

This assay is FDA cleared for quantitative EBV DNA testing on plasma and its performance has been evaluated by the Barnes-Jewish Hospital Molecular Infectious Disease Laboratory.

CPT:  87799

Reference Values

Not detected.

Specimen Transport Instructions

Whole blood specimens should be delivered to the BJH Molecular Infectious Disease laboratory within 4 hours of collection for processing.

Specimen Stability Information

Specimens may be stored under the following conditions:

Prior to separation:

  • 2 to 25°C for up to 24 hours

Following separation of plasma from whole blood:

  • Room temperature for up to 24 hours
  • 2 to 8°C for up to 6 days
  • -20°C to -80°C for up to 6 months

 

Additional Information

Assay Limit of Detection - Plasma: 18.8 IU/mL

Assay Quantitative Range - Plasma: 35 IU/mL - 1.0x108 IU/mL

For BJH Laboratory Use Only

Laboratory Processing Instructions        

Specimens should be delivered to the BJH MID laboratory. Specimen is stored frozen at the BJH MID lab for 21 days.