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HelpTest Code LAB6532 Immunofixation Electrophoresis, Urine
Performing Laboratory
Barnes-Jewish Hospital Laboratory
Methodology
Gel Electrophoresis/Immunoprecipitation
Note: This test is automatically performed when #5309 Protein Electrophoresis, 24 Hour, Urine is ordered.
Literature Reference:
Whicher JT, Hawkins L, Higginson J. Clinical applications of immunofixation: a more sensitive technique for the detection of Bence-Jones protein. J Clin Pathol 988;33:779-80.
Specimen Requirements
Submit only 1 of the following specimens:
Preferred
Specimen Type: Urine, 24 hour
Container/Tube: Plain brown urine container
Specimen Volume: Entire collection
Collection Instructions: No additive. Refrigerate specimen during collection.
Additional Information:
1. Starting and ending times of collection are required.
2. See Urine Collection in Special Instructions for additional information.
3. Collection periods <20 hours will be ordered as a timed-urine collection.
4. Collection periods >48 hours require approval from the Laboratory Medicine resident before analysis.
Specimen Type: Urine, random
Container/Tube: Urine Complete Cup Kit
Specimen Volume: 20 mL from a first-morning collection.
Collection Instructions: No additive.
Additional Information: See Urine Collection in Special Instructions for additional information.
Alternate
Specimen Type: Urine, timed collection
Container/Tube: Plain brown urine container
Specimen Volume: Entire collection
Collection Instructions: No additive. Refrigerate specimen during collection.
Additional Information:
1. Starting and ending times of collection are required.
2. See Urine Collection in Special Instructions for additional information.
3. Collection periods <20 hours will be ordered as a timed-urine collection.
4. Collection periods >48 hours require approval from the Laboratory Medicine resident before analysis.
Day(s) Test Set Up
Monday through Friday
Cut-off time: 0800
Turnaround Time:
STAT: not available
Routine: 2 working days
Test Classification and CPT Coding
86335
Reference Values
No monoclonal protein detected.
Literature Reference:
Alper CA, Johnson AM. Immunofixation electrophoresis: a technique for the study of protein polymorphism. Vox Sang 1969;17:445-52.
Specimen Transport Temperature
Ambient
Additional Information
For BJH Laboratory Use Only
Alternate Tube:
Plain, brown urine container preferred; any clean container will be accepted.
Analyte Stability:
24 hours at ambient temperature is acceptable; refrigeration during collection is preferred.
Laboratory Processing Instructions:
Test performed in BJH Core Lab. If ordered with a urine protein electrophoresis; this is a duplicate and should be cancelled. If ordered alone; do not send an aliquot to Chemistry for total protein, and concentrate 100x. Keep a 10-mL urine specimen in refrigerator for possible future use. Place concentrate in appropriate rack.
Special Instructions
LOINC Code Information
| Name | LOINC Code |
| Immunofixation, Urine Interpretation | 14896-5 |