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HelpTest Code Lab4010 Obstetric Panel with HIV
Performing Laboratory
Barnes-Jewish Hospital Laboratory
Methodology
| Profile Information: | |
| Complete Blood Count (CBC) | Rubella Antibodies, IgG |
| Hepatitis Bs Antigen (HBsAg) | Type and Screen |
| Rapid Plasma Reagin (RPR), Qualitative | HIV-1/HIV-2 Ab + p24 Ag |
Method Name:
See individual test listings.
Testing Algorithm:
If HBsAg is positive, then hepatitis Bs antigen confirmation will be performed, at an additional charge. If antibody screen is positive, then antibody identification and #9874 Antibody Titration, Blood may be performed, at an additional charge.
If HIV reactive, antibody differentiation testing is performed. If the differentiation test is reactive for HIV-1 antibodies only, or reactive for HIV-1 and HIV-2 antibodies, or is not reactive for HIV-1 and HIV-2 antibodies. HIV-1 RNA (viral load) test is recommended to be performed with a separate specimen at BJH Molecular Diagnostics Laboratory. If the differentiation test is reactive for HIV-2 antibodies, HIV-2 PCR can be considered with the assistance of the LMR, since HIV-2 infection is rare in the United States. The patient's history and clinical condition can be considered if HIV-2 PCR testing is required.
Specimen Requirements
2 ETDA tubes, 1 Pink top, 1 Mint green tube, and 1 Plain red top tube are required.
Hospital Patient
CBC
Specimen Type: Whole blood
Container/Tube: Lavender top (EDTA)
Specimen Volume: Full tube
Collection Instructions: Immediately invert several times to mix blood.
Additional Information: Label specimen appropriately (blood for CBC).
Type and Screen
Specimen Type: Whole blood
Container/Tube: Pink top (EDTA)-Separator gel tube is not acceptable.
Specimen Volume: Full tube
Additional Information:
1. Specimen must be labeled with barcode label or addressograph imprint including patient’s name, hospital registration number, and date of birth.
2. Specimen must have 2 signatures (phlebotomist and witness). Initials are not acceptable.
HBsAg
Specimen Type: Serum
Container/Tube: Plain red top
Specimen Volume: Full tube
Additional Information: Label specimen appropriately
Rubella
Specimen Type: Plasma
Container/Tube: Mint green tube
Specimen Volume: Full tube
Additional Information: label specimen appropriately
RPR
Specimen Type: Serum
Container/Tube: Plain red top
Specimen Volume: Full tube
Additional Information: Label specimen appropriately (serum for RPR).
HIV-1/HIV-2 Ab +p24 Ag
Specimen Type: Plasma
Container/Tube: Pink top tube
Specimen Volume: Full tube (7mL)
Non Hospital Patient:
CBC
Specimen Type: Whole blood
Container/Tube: Lavender top (EDTA)
Specimen Volume: Full tube
Collection Instructions: Immediately invert several times to mix blood.
Additional Information: Label specimen appropriately (blood for CBC).
Type and Screen
Specimen Type: Whole blood
Container/Tube: Pink top (EDTA)-Separator gel tube is not acceptable.
Specimen Volume: Full tube
Additional Information:
1. Label tube with patient’s name, date of birth, and Social Security number.
2. Specimen must be signed by person drawing the specimen. Initials are not acceptable.
3. If a type and screen is also requested or is added to this request, the specimen must have 2 signatures (phlebotomist and witness). Initials are not acceptable. Specimens without 2 signatures may be tested but will not be used for crossmatch purposes.
HBsAg
Specimen Type: Serum
Container/Tube: Plain red top
Specimen Volume: Full tube
Additional Information: Label specimen appropriately
Rubella
Specimen Type: Plasma
Container/Tube: Mint green tube
Specimen Volume: Full tube
Additional Information: Label specimen appropriately
RPR
Specimen Type: Serum
Container/Tube: Plain red top
Specimen Volume: Full tube
Additional Information: Label specimen appropriately (serum for RPR).
HIV-1/HIV-2 Ab + p24 Ag
Specimen Type: Plasma
Container/Tube: Pink top tube
Specimen Volume: Full tube (7mL)
Day(s) Test Set Up
See individual test listings.
Test Classification and CPT Coding
80081-Obstetric panel with HIV-1/HIV-2 Ab + p24 Ag
86870-Antibody identification (if appropriate)
86886-Antibody titration (if appropriate)
86701--HIV-1 Multispot (if appropriate)
86702--HIV-2 Multispot (if appropriate)
87536--HIV-1 RNA (if appropriate)
Reference Values
See individual test listings.
Specimen Transport Temperature
Ambient
Additional Information
For BJH Laboratory Use Only
Alternate Tube:
See individual test listings.
Analyte Stability:
See individual test listings.
Laboratory Processing Instructions:
Tests performed in BJH Blood Bank and Core Lab. See individual test listings.
Note: Upon confirmation, all positive HBsAg results must be reported to the state within 7 days.
Confirmed HIV-1 positives are reported to the state within 7 days.