Test Code Lab6558 UA Reflex Microscopic (Urine Macroscopic with Reflex Microscopic, if Indicated)

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Methodology

Testing Algorithm:

If there are abnormal dipstick findings (protein ≥+1, any blood, nitrite, or leukocyte esterase), then #LAB348 Urine Microscopic will be performed at an additional charge.

Method Name:

Dipstick: Spectrophotometric reflectance of light on color reagent pads

Urine color: Spectrophotometry or visual observation

Specific gravity: Refractometry

Clarity: Measurement of the transmission and scatter of light

Includes color, clarity, specific gravity, pH, protein, glucose, ketones, bilirubin, occult blood, urobilinogen, nitrites, and leukocyte esterase.

Literature References:

AUTION MAX^TM AX-4030 Operating Manual. ARKRAY, Inc. 5182 W 76^th St., Minneapolis, MN May,2019 PN 84-02160K
Instructions for Use IQ200 Series. Beckman Coulter. Lismeehan O’Callahan’s Mills CO Clare Ireland. July, 2020.PN:300-4320CE
AUTION Sticks 9EB. ARKRAY, Inc. 5182 W 76^th St., Minneapolis, MN May,2019 PN:CP73501-010D
Brunzel, N. A.: Fundamentals of Urine and Body Fluid Analysis, 4^th ed., St. Louis, MO; Elsevier; 2018; pp. 68-210.

Specimen Requirements

Specimen Type: Urine, random

Container/Tube: UA preservative tube(Yellow/Red)

Specimen Volume: Full tube Specimen Minimum Volume: 5 mL

Collection Instructions:

1. Collect a midstream voided specimen.Urine Collection

3. Using cannula in collection cup lid, fill yellow-top, plastic, conical tube.

Additional Information:

1. Label tube with patient’s name and date of birth.

2. A catheterized specimen is acceptable.

3. Provide collection date and time, and specimen source (method of collection).

4. If storage and transport is >1 hour, refrigerate specimen.

5. Specimen should be received in the laboratory within 2 hours of collection.

Day(s) Test Set Up

Monday through Sunday, 24 hours / 7 days a week

Turnaround Time:

Urinalysis: STAT: 1 hour after receipt in laboratory

Routine: 2-4 hours after receipt in laboratory

Test Classification and CPT Coding

81003-Urine macroscopic

81015-Urine microscopic (if appropriate)

Reference Values

Specific gravity: 1.003-1.030
pH: "Urine pH is affected by diet, medications, systemic acid-base disturbances, and renal tubular function. pH may affect urinary stone formation. For example, urine pH below 6.0 may help reduce the tendency for calcium phosphate stones and pH greater than 6.0 may reduce the tendency for uric acid stone formation.
Information obtained from Mayo Medical Laboratories test catalog 2/20/18."
Protein: negative
Glucose: negative
Ketones: negative
Bilirubin: negative
Occult blood: negative
Urobilinogen: <2.0 mg/dL
Nitrites: negative
Leukocyte esterase: negative

Specimen Transport Temperature

Ambient <1 hour

Refrigerate >1 hour

Additional Information

For BJH Laboratory Use Only

Alternate Tube(s):

1 Yellow top urine tube

Analyte Stability:

Specimen should be received in the laboratory within 2 hours of collection. However, using a UA Preservative tube extends the stability

Laboratory Processing Instructions:

If specimen is received in a cup, lab processing personnel should use the cannula in the lid to transfer the urine to a yellow top tube before transporting to the UA tech.

Test performed in BJH Core Lab. Performed on uncentrifuged urine. If there are abnormal dipstick findings (protein ≥ +1, any blood, nitrite, or leukocyte esterase), a urine microscopic exam will be performed.

LOINC Codes

Macroscopic

Name LOINC code

Color	50553-7
Clarity	32167-9
pH Ur	50560-2
Specific Gravity Ur	53326-5
Protein Ur	50561-0
Blood Ur	50559-4
Glucose Ur	53328-1
Ketones Ur	50557-8
Leuk Esterase Ur	60026-2
Nitrite Ur	50558-6
Urobilinogen Ur	50563-6

Microscopic